D3514
 |  |  |
 |
Image
D3514
Sigma
4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate
≥80% (elemental analysis), powder
| Synonym: | DIDS, Disodium 4,4′-diisothiocyanatostilbene-2,2′-disulfonate |
| CAS Number: | 207233-90-7 |
| Linear Formula: | C16H8N2Na2O6S4 · xH2O |
| Molecular Weight: | 498.48 (anhydrous basis) |
| Beilstein Registry Number: | 8179433 |
| MDL number: | MFCD00009638 |
| PubChem Substance ID: | 24893761 |
Description
| Application | A negatively charged homobifunctional cross-linking reagent. The isothiocyanate groups react with primary amines at pH 9.0-10.0. |
| Biochem/physiol Actions | A specific inhibitor of cellular anion permeability used in cell transport studies. |
Properties
| assay | ≥80% (elemental analysis) |
| form | powder |
| color | yellow |
| solubility | 0.1 M potassium bicarbonate: 50 mg/mL |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-26 |
| WGK Germany | 3 |
References
| reference | Okada, Y., et al., Volume-sensitive chloride channels involved in apoptotic volume decrease and cell death. J. Membr. Biol. 209, 21-29, (2006) |
| Pusch, M., et al., Channel or transporter? The CLC saga continues. Exp. Physiol. 91, 149-152, (2006) | |
| Alper, S.L., Molecular physiology of SLC4 anion exchangers. Exp. Physiol. 91, 153-161, (2006) | |
| Hua, S., and Inesi, G., Lys515-Lys492 cross-linking by DIDS interferes with substrate utilization by the sarcoplasmic reticulum ATPase. Biophys. J. 73, 2149-2155, (1997) | |
| Kavanaugh, M.P., et al., Affinity labeling of hemoglobin with 4,4'-diisothiocyanostilbene-2,2'-disulfonate: covalent cross-linking in the 2,3-diphosphoglycerate binding site. Biochemistry 27, 1804-1808, (1988) |
